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pad track flag sirt1 h355a  (Addgene inc)
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Addgene inc pad track flag sirt1 h355a
Pad Track Flag Sirt1 H355a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pad+track+sirt1+h355a/pmc05081691-462-20-23?v=Addgene+inc
Average 91 stars, based on 1 article reviews
pad track flag sirt1 h355a - by Bioz Stars, 2026-08
91/100 stars
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pad track sirt1 h355a  (Addgene inc)
90
Addgene inc pad track sirt1 h355a
FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or <t>SIRT1(H355A).</t> Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.
Pad Track Sirt1 H355a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pad+track+sirt1+h355a/10__1074_slash_jbc__m110__123620-70-21-33?v=Addgene+inc
Average 90 stars, based on 1 article reviews
pad track sirt1 h355a - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

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FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or SIRT1(H355A). Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.

Journal: Journal of Biological Chemistry

Article Title: Macrophage α1 AMP-activated Protein Kinase (α1AMPK) Antagonizes Fatty Acid-induced Inflammation through SIRT1

doi: 10.1074/jbc.m110.123620

Figure Lengend Snippet: FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or SIRT1(H355A). Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.

Article Snippet: Plasmid Constructs and Transfection—Construction of constitutively active (CA) and dominant-negative (DN) 1AMPK expression vectors were described previously (20). pCruzSIRT1, pAd-Track SIRT1, pAd-Track-SIRT1 H355A, SIRT2SIRT7, p300, and p65 expression vectors were all purchased from Addgene (Cambridge, MA).

Techniques: Expressing, Western Blot, Activation Assay, Transfection, Knockdown, Control, Activity Assay